Human Phospho-IRS1 (Y612) Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB7314N Datasheet / COA / SDS

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FAB7314N-100UG

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Human Phospho-IRS1 (Y612) Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRS1 when phosphorylated at Y612 in direct ELISAs.

Source

Monoclonal Mouse IgG2a Clone # 738662

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human IRS1 Y612 site
Accession # P35568

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Phosphorylation on Y612 and Y632 is important for IRS1 to fully activate PI3K and translocate GLUT4 in respose to insulin. The sequence surrounding pTyr612 is identical between human and mouse IRS1.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1