Human phospho-NFATC1 (S172) Antibody (679340)

R&D Systems | Catalog # MAB5640

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 679340
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human S172 site.

Specificity

Detects human NFATC1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human phospho-NFATC1 (S172) Antibody (679340)

Detection of Phospho-NFATC1 (S172) by Western Blot

Detection of Phospho-NFATC1 (S172) by Western Blot

Obestatin promotes activation of NFTAc1. a Comparation of the effect of obestatin (10 nM) and insulin (1.72 µM) on utrophin, beta -dystroglycan, and alpha 7-integrin protein expression on differentiating human DMD cells. b Analysis of pNFATc1(S172) and NFATc1 after obestatin (10 nM) or insulin (1.72 µM) administration on differentiating human DMD cells. c Representative immunofluorescence images of obestatin- or control-treated human DMD myotubes showing NFATc1 cellular location. Right panel, measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (*P < 0.05). d Representative images of vehicle- and obestatin-treated TAs showing NFATc1 expression. Right panel, measurement of the number of nuclei showing NFATc1 location. Data are shown as mean ± SEM (*P < 0.05). e Immunoblot analysis of PPP3 catalytic subunit (PPP3CA), pNFATc1(S172), NFATc1, utrophin, slow-MHC, fast-MHC, beta -dystroglycan, alpha -syntrophin, NOS1, beta 1D-integrin, and alpha 7-integrin in extracts of DMD cells transfected with control or PPP3CA siRNAs after obestatin treatment (10 nM). The inset shows Proposed model by which obestatin signalling triggers PPP3 to regulate the transcriptional activity of NFATc1. Data were expressed as mean ± SEM (n = 3 per group; *,#P < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41288941), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human phospho-NFATC1 (S172) Antibody (679340)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Reviewed Applications

Read 4 reviews rated 4 using MAB5640 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca++, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human); 18018 (Mouse)

Gene Symbol

NFATC1

Additional NFATC1 Products

Product Documents for Human phospho-NFATC1 (S172) Antibody (679340)

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Product Specific Notices for Human phospho-NFATC1 (S172) Antibody (679340)

For research use only

Citations for Human phospho-NFATC1 (S172) Antibody (679340)

Customer Reviews for Human phospho-NFATC1 (S172) Antibody (679340) (4)

4 out of 5
4 Customer Ratings
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4 Stars
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Showing  1 - 4 的 4 reviews Showing All
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  • Human Phospho-NFATC1 (S172) Antibody
    Name: Nar Katuwal
    Application: Western Blot
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 04/21/2021
    Human phospho-NFATC1 (S172) Antibody (679340) MAB5640
  • Name: Jignesh Ganeshbhai Patel
    Application: Western Blot
    Sample Tested: HeLa cells and Blood monocytes
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa cells and THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Human Phospho-NFATC1 (S172) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Skeletal muscle
    Species: Mouse
    Verified Customer | Posted 07/19/2017
    Overloaded plantaris muscles have greater calcineurin signalling and less phosphorylated NFATc1 (~120 kDa). **The band at ~80 kDa may represent the dephosphorylated form of NFATc1.
    Human phospho-NFATC1 (S172) Antibody (679340) MAB5640

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