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Human Phospho-Smad2/3 (S465/S467) Antibody

Catalog #: MAB8935
13 Citations 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
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MAB8935
MAB8935-SP

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Detection of Human Phospho-Smad2/3 (S465/S467) by Western Blot.
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Citations (13)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Smad2/3 (S465/S467) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smad2 and Smad3 when dually phosphorylated at S465 and S467 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1074A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human Smad2 S465/S467 site
Accession # NP_005892
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Smad2/3 (S465/S467) antibody by Western Blot. View Larger

Detection of Human Phospho-Smad2/3 (S465/S467) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad2/3

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Long Name
Mothers Against DPP Homolog 2/3
Alternate Names
hMAD-2; hSMAD2; JV18; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2MGC34440; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smad2/3

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Citations for Human Phospho-Smad2/3 (S465/S467) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway
    Authors: Yoshida M, Kokubun T, Sato K et al.
    Investigative ophthalmology & visual science
  2. Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGF?1 Pathway
    Authors: Passanha FR, Geuens T, LaPointe VLS
    Stem cells (Dayton, Ohio)
  3. Persistent neutrophilic inflammation is associated with delayed toxicity of phenylarsine oxide in lungs
    Authors: Sanchez, NC;Mani, G;Nowak, JI;Jones, C;Kim, YI;Sharma, NS;Zmijewski, JW;Surolia, R;
    Scientific reports
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  4. Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-&beta1-Induced Intestinal Fibrosis on CCD-18Co Cells
    Authors: F Lombardi, FR Augello, P Palumbo, E Mollsi, M Giuliani, AM Cimini, MG Cifone, B Cinque
    Nutrients, 2021-03-09;13(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Hypoxia-induced TGF-beta –RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer
    Authors: Neha Ahuja, Cheemala Ashok, Subhashis Natua, Deepak Pant, Anna Cherian, Madhura R Pandkar et al.
    NAR Cancer
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP (chromatin immunoprecipit
  6. Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles
    Authors: LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka
    Nat Commun, 2020-05-11;11(1):2331.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
    Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
    Cell Rep, 2019-12-10;29(11):3488-3505.e9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  8. CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro
    Authors: S Zhou, SD da Silva, PM Siegel, A Philip
    Sci Rep, 2019-11-06;9(1):16317.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  9. Label-free quantitative proteomics identifies transforming growth factorβ1 (TGF-?1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells
    Authors: J Tan, Y Wang, S Wang, S Wu, Z Yuan, X Zhu
    Cell Biosci, 2019-06-14;9(0):48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  10. COA-Cl prevented TGF-?1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis
    Authors: K Nakai, S Karita, J Igarashi, I Tsukamoto, K Hirano, Y Kubota
    J. Dermatol. Sci., 2019-03-12;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation
    Authors: Tao Cheng
    Am. J. Pathol, 2016-12-08;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK
    Authors: Yinmin Wang, Xiuxia Wang, Xianyu Zhou, Zhu Zhu, Jun Yang, Fei Liu
    International Journal of Molecular Medicine
  13. Hypoxia-induced TGF-beta –RBFOX2–ESRP1 axis regulates human MENA alternative splicing and promotes EMT in breast cancer
    Authors: Neha Ahuja, Cheemala Ashok, Subhashis Natua, Deepak Pant, Anna Cherian, Madhura R Pandkar et al.
    NAR Cancer

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Human Phospho-Smad2/3 (S465/S467) Antibody
By Anonymous on 07/08/2020
Application: WB Sample Tested: Mv1Lu mink lung epithelial cell line Species: Mink
Western Blot Human Phospho-Smad2/3 (S465/S467) Antibody MAB8935