Human PLD1 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PLD1

PLD1 (Phospholipase D1a) is a 110‑120 kDa member of the phospholipase D family of enzymes. It is expressed in endothelial cells and select tissues. Following activation and association with Type I alpha PIPkinase, PLD1 hydrolyzes the phosphodiester bond of membrane phosphatidylcholine, generating phosphatidic acid. Human PLD1 is 1074 amino acids in length). It contains one PX/phox homology domain (aa 84‑206), a pleckstrin homology domain (aa 219‑328), and two phosphodiesterase enzyme regions (aa 459‑486 and 891‑918). There are three splice variants. One shows a 10 aa substitution for aa 962‑1074 (PLD1d), a second shows an 84 aa substitution for aa 514‑1074 (PLD1c), and a third shows an Asn substitution for aa 585‑623 (PLD1b). Caspase cleavage of PLD1a at Gln545‑Ser546 generates a 62 kDa N-terminal, and a 61 kDa C-terminal fragment. Over aa 1‑140, human PLD1a shares 91% aa identity with mouse PLD1a.

Product Datasheets

Related Research Areas

• Lipid/Cholesterol Metabolism Enzymes
• Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway