Human PLD1 Antibody

Catalog #: AF5615 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5615
AF5615-SP

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Detection of Human PLD1 by Western Blot.

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Human PLD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PLD1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLD1
Met1-Pro140
Accession # Q13393

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PLD1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PLD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5615) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLD1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLD1

PLD1 (Phospholipase D1a) is a 110‑120 kDa member of the phospholipase D family of enzymes. It is expressed in endothelial cells and select tissues. Following activation and association with Type I alpha PIPkinase, PLD1 hydrolyzes the phosphodiester bond of membrane phosphatidylcholine, generating phosphatidic acid. Human PLD1 is 1074 amino acids in length). It contains one PX/phox homology domain (aa 84‑206), a pleckstrin homology domain (aa 219‑328), and two phosphodiesterase enzyme regions (aa 459‑486 and 891‑918). There are three splice variants. One shows a 10 aa substitution for aa 962‑1074 (PLD1d), a second shows an 84 aa substitution for aa 514‑1074 (PLD1c), and a third shows an Asn substitution for aa 585‑623 (PLD1b). Caspase cleavage of PLD1a at Gln545‑Ser546 generates a 62 kDa N-terminal, and a 61 kDa C-terminal fragment. Over aa 1‑140, human PLD1a shares 91% aa identity with mouse PLD1a.

Long Name

Phospholipase D1

Entrez Gene IDs

5337 (Human); 18805 (Mouse); 25096 (Rat)

Alternate Names

Choline phosphatase 1; EC 3.1.4.4; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; phospholipase D1; phospholipase D1, phophatidylcholine-specific; phospholipase D1, phosphatidylcholine-specific; PLD 1; PLD1