Human POT1 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5299U Datasheet / COA / SDS

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AF5299U-100UG

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Human POT1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human POT1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human POT1
Met159-Pro316
Accession # Q9NUX5

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: POT1

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1‑300) and a C-terminal TPP-1 dimerization domain (aa 301‑634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43‑634, a 24 aa substitution for aa 317‑634, a three aa substitution for aa 457‑634, and a 16 aa substitution for aa 503‑634. Over aa 159‑316, human POT1 shares 71% aa identity with mouse POT1.

Long Name

Protection of Telomeres Protein 1

Entrez Gene IDs

25913 (Human); 101185 (Mouse); 500054 (Rat)

Alternate Names

DKFZp586D211; HPOT1; POT1 protection of telomeres 1 homolog; POT1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog (S. pombe); protection of telomeres protein 1