POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1‑300) and a C-terminal TPP-1 dimerization domain (aa 301‑634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43‑634, a 24 aa substitution for aa 317‑634, a three aa substitution for aa 457‑634, and a 16 aa substitution for aa 503‑634. Over aa 159‑316, human POT1 shares 71% aa identity with mouse POT1.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Met159-Pro316
Accession # Q9NUX5
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human POT1 Antibody
Detection of Human POT1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human POT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POT1 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Applications for Human POT1 Antibody
Western Blot
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: POT1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional POT1 Products
Product Documents for Human POT1 Antibody
Product Specific Notices for Human POT1 Antibody
For research use only
Citations for Human POT1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars