Human/Rat Annexin V Antibody

Detection of Human Annexin V by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Annexin V by Simple Western^TM. Simple Western lane view shows lysates of human lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 39 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Rat Annexin V Monoclonal Antibody (Catalog # MAB399). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human Annexin V Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Annexin V knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Rat Annexin V Monoclonal Antibody (Catalog # MAB399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.