Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cortactin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cortactin
Met1-Gly85
Accession # Q14247

Specificity

Detects human and rat Cortactin in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat Cortactin Antibody

Detection of Human and Rat Cortactin antibody by Western Blot.

Detection of Human and Rat Cortactin by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, MDA-MB-453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Cortactin at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Cortactin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Cortactin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cortactin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cortactin at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Rat Cortactin Antibody

Application
Recommended Usage

Knockout Validated

Cortactin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cortactin knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line, MDA‑MB‑453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cortactin

Cortactin (also EMS1 and Amplaxin) is an 80‑85 kDa multidomain scaffold-associated protein. It is an actin-binding cytoplasmic protein found at intercellular junctions and near lamellipodia. It is also found in tumor invadopodia, a somewhat analogous structure to lamellipodia. Here, Cortactin appears to coordinate actin assembly with MMP secretion, thus facilitating tumor invasiveness. Human Cortactin is 550 amino acids (aa) in length and contains six-plus Cortactin regions (aa 80‑324) that regulate actin polymerization, and one SH3 domain (aa 496‑548) that binds to N-WASP. Cortactin is highly phosphorylated on Tyr and Ser/Thr, and undergoes acetylation.

Alternate Names

Amplaxin, CTTN, EMS1

Entrez Gene IDs

2017 (Human); 13043 (Mouse); 60465 (Rat)

Gene Symbol

CTTN

UniProt

Q14247 (Human)

Additional Cortactin Products

Product Documents for Human/Rat Cortactin Antibody

Certificate of Analysis

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Product Specific Notices for Human/Rat Cortactin Antibody

For research use only

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