Histone Deacetylase 2/HDAC2 Antibody (806116)

R&D Systems | Catalog # MAB7679

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 806116
View all Histone Deacetylase 2/HDAC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Histone Deacetylase 2/HDAC-2
Pro386-Pro488
Accession # Q92769

Specificity

Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (806116)

Detection of Human and Rat Histone Deacetylase 2/HDAC2 antibody by Western Blot.

Detection of Human and Rat Histone Deacetylase 2/HDAC2 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Histone Deacetylase 2/HDAC2 antibody by Simple WesternTM.

Detection of Human Histone Deacetylase 2/HDAC2 by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Rat Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Histone Deacetylase 2/HDAC2 Antibody (806116)

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Western Blot

0.2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 4 using MAB7679 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), is a ubiquitously expressed intracellular enzyme that is involved in the post-translational modification of proteins. Acetyl groups are added to e-NH3 on lysine residues by acetyltransferases and removed by HDACs. The balance between these reactions has widespread effects on the enzymatic activity, subcellular localization, and molecular interactions of the substrates. HDAC2 can associate into several multiprotein complexes with discrete functions. Acetylation of core histones (H2A, H2B, H3, and H4) alters their interaction with DNA and the local availability of DNA for transcription. The function of HDAC2 itself is regulated by several post-translational modifications including phosphorylation, acetylation, ubiquitination, tyrosine nitrosylation, and cysteine carbonylation. Within aa 386-488, human HDAC2 shares 98% and 96% aa sequence identity with mouse and rat HDAC2, respectively.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Entrez Gene IDs

3066 (Human); 15182 (Mouse); 84577 (Rat)

Gene Symbol

HDAC2

UniProt

Q92769 (Human)

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (806116)

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (806116)

For research use only

Citations for Histone Deacetylase 2/HDAC2 Antibody (806116)

Customer Reviews for Histone Deacetylase 2/HDAC2 Antibody (806116) (1)

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  • Human/Rat Histone Deacetylase 2/HDAC2 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 01/23/2023
    This experiment was conducted under reducing conditions, using the 12-230 kDa separation system, Jurkat whole cell lysates (loaded at 0.6 mg/ml protein concentration), HDAC2 antibody 1:100 (Catalog # MAB7679). Multiple bands detected: 65, 100 and 141 kDa.
    Histone Deacetylase 2/HDAC2 Antibody (806116) MAB7679

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Associated Pathways

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