Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RGM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RGM-A
Cys48-Gly422
Accession # Q96B86

Specificity

Detects human RGM-A in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human RGM‑A Antibody

RGM-A antibody in Human Brain by Immunohistochemistry (IHC-P).

RGM‑A in Human Brain.

RGM-A was detected in immersion fixed paraffin-embedded sections of human brain (spinal cord) using Goat Anti-Human RGM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2459) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of RGM-A by Western Blot

Detection of RGM-A by Western Blot

HFE2 administration significantly improves EAE disease outcome. A Western Blot analysis shows a strong HFE2 reduction in EAE animal serum when compared to control. ELISA quantification showing HFE2 decrease and RGMa increase in EAE animal serum (mean ± s.d.; EAE n = 3 and RGMa n = 3). b Body conditioning assessment (‘0’ no paralysis to ‘4’ forelimb weakness) of EAE-induced mice with HFE2 + /- RGMa treatment every 3 days (d3, 6, 9, 12 and 15; mean ± s.e.m.; unpaired two-tail t-tests; PBS n = 8, HFE2 n = 6, RGMa+ HFE2 n = 8). c Body conditioning assessment of EAE-induced Hfe2fl/fl and Hfe2 delta Alb-cre mice (mean ± s.e.m.; unpaired two-tail t-tests; Hfe2fl/fln = 8 and Hfe2 delta Alb-cren = 8). d Representative confocal images of CD3 +, B220+ and CD11b+ immune staining in spinal cord of EAE-induced mice 3 weeks after induction and quantification (mean ± s.e.m.; unpaired two-tail t-test; PBS n = 7, HFE2 n = 6 mice; technical replicates shown). e Schematic of activated immune cell adoptive transfer. Representative confocal images of CD4-positive labelled transferred cell (CellVue) in PBS-treated or HFE2-treated recipient WT mice and quantification (mean ± s.e.m.; unpaired two-tail t-test; PBS n = 6, HFE2 n = 6). Ptx; Pertussis Toxin. f Representative confocal images of fibrinogen in the spinal cord of HFE2-treated EAE-mice and quantification (mean ± s.e.m.; unpaired two-tail t-test; PBS-treated n = 6 and HFE2 n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file (includes exact p-values). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-45303-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human RGM‑A Antibody

Application
Recommended Usage

Immunohistochemistry

10-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human spinal cord subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Repulsive Guidance Molecule A/RGM-A (Catalog # 2459-RM)

Reviewed Applications

Read 1 review rated 5 using AF2459 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RGM-A

Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).

References

  1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  2. Monnier P. et al. (2002) Nature 419:392.
  3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  7. Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Q96B86

Additional RGM-A Products

Product Documents for Human RGM‑A Antibody

Certificate of Analysis

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Product Specific Notices for Human RGM‑A Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human RGM‑A Antibody

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  • Human RGM-A Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 02/04/2025
    Worked in detecting 2459-RM in in vitro assay
    Human RGM‑A Antibody AF2459

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