Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 374521
View all RSK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RSK4
Met1-Leu745
Accession # Q9UK32

Specificity

Detects human RSK4 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) RSK1, rhRSK2, or rhRSK3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human RSK4 Antibody

Detection of Human RSK4 antibody by Western Blot.

Detection of Human RSK4 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human RSK4 antibody by Western Blot.

Detection of Human RSK4 by Western Blot.

Western blot shows Recombinant Human Active RSK1 (Catalog # 992-KS), recombinant human RSK2, recombinant human RSK3, and recombinant human RSK4 (2 ng/lane). PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RSK4 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RSK4

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases frequently activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSK4, also known as RPS6KA6, is commonly deleted in patients with complex X-linked mental retardation. Unlike other RSKs, RSK4 is constitutively active in serum-starved cells, and may participate in non-growth factor signaling.

Long Name

Ribosomal Protein S6 Kinase IV

Alternate Names

RPS6KA6

Entrez Gene IDs

27330 (Human); 67071 (Mouse); 317203 (Rat)

Gene Symbol

RPS6KA6

UniProt

Q9UK32

Additional RSK4 Products

Product Documents for Human RSK4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RSK4 Antibody

For research use only

Citations for Human RSK4 Antibody

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