Human SPRY1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6097V Datasheet / COA / SDS

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FAB6097V-100UG

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Human SPRY1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SPRY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SPRY2, 3, or 4 is observed.

Source

Monoclonal Mouse IgG2b Clone # 669534

Purification

Protein A or G purified

Immunogen

E.coli-derived recombinant human SPRY1
Met1-Lys178
Accession # O43609

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SPRY1

SPRY1 (sprouty homolog 1) is a 34-38 kDa member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gamma 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 amino acids (aa) in length. It contains one CBL-TKB binding site (aa 51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa 112-131), and a Cys-rich domain (aa 181-306) that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa 1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.

Long Name

Sprouty Homolog 1

Entrez Gene IDs

10252 (Human); 24063 (Mouse); 294981 (Rat)

Alternate Names

Drosophila, homolog of, 1 (antagonist of FGF signaling); sprouty homolog 1, antagonist of FGF signaling (Drosophila); SPRY1; spry-1