Human TCF-12/HTF4 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB6928T Datasheet / COA / SDS

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FAB6928T-100UG

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All TCF-12/HTF4 Antibodies

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Human TCF-12/HTF4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCF-12/HTF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TCF1, -2, -3, or -5 is observed.

Source

Monoclonal Mouse IgG2b Clone # 690124

Purification

IgM-specific Affinity-purified

Immunogen

E. coli-derived recombinant human TCF-12/HTF4
Met108-Glu521
Accession # Q99081

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TCF-12/HTF4

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Long Name

Transcription Factor 12

Entrez Gene IDs

6938 (Human); 21406 (Mouse); 25720 (Rat)

Alternate Names

BHLHB20; bHLHb20E-box-binding protein; Class B basic helix-loop-helix protein 20; HEB; HEBDNA-binding protein HTF4; helix-loop-helix transcription factor 4; HsT17266; HTF4; HTF4TCF-12; TCF12; TCF-12; transcription factor 12; Transcription factor HTF-4