Human TCF-12/HTF4 Antibody (690124)

R&D Systems | Catalog # MAB6928

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 690124
View all TCF-12/HTF4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TCF-12/HTF4
Met108-Glu521
Accession # Q99081

Specificity

Detects human TCF-12/HTF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TCF1, -2, -3, or -5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TCF-12/HTF4 Antibody (690124)

Detection of Human TCF-12/HTF4 antibody by Western Blot.

Detection of Human TCF-12/HTF4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB6928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF-12/HTF4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TCF-12/HTF4 Antibody (690124)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCF-12/HTF4

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Entrez Gene IDs

6938 (Human); 21406 (Mouse); 25720 (Rat)

Gene Symbol

TCF12

UniProt

Q99081

Additional TCF-12/HTF4 Products

Product Documents for Human TCF-12/HTF4 Antibody (690124)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TCF-12/HTF4 Antibody (690124)

For research use only

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