Human Testican 1/SPOCK1 Antibody (316801)

R&D Systems | Catalog # MAB2327

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 316801
View all Testican 1/SPOCK1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
Arg22-Trp439
Accession # Q08629

Specificity

Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Testican 1/SPOCK1 Antibody (316801)

Detection of Testican 1/SPOCK1 by Immunohistochemistry

Detection of Testican 1/SPOCK1 by Immunohistochemistry

Identification of SPOCK1 as a prognostic key gene within the key modules. A Venn plot of the intersection of differentially expressed genes (DEGs) that are abnormally upregulated in tumors and genes within the red and green modules. B Determination of the optimal lambda when the partial likelihood of deviance reaches a minimum. And LASSO coefficient of module candidate gene profiles. C Screening of the top 10 prognosis-related genes in terms of relative importance using the stochastic survival forest algorithm. D Intersection Venn plot of prognostic key genes. Hub genes’ correlation heatmap with (E) EMT score and (F) TIDE score. (***p < 0.001; **p < 0.01; *p < 0.05; ns. no significance) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38087364), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Testican 1/SPOCK1 by Immunohistochemistry

Detection of Testican 1/SPOCK1 by Immunohistochemistry

SPOCK1 is associated with invasive properties. In the GSE166722 dataset, (A) SPOCK1 expression levels in invasive phenotype and inert phenotype LUAD. B ROC curves showing the diagnostic efficacy of SPOCK1 in differentiating between aggressive and inert patients. C Differential expression levels of SPOCK1 in different aggressive pathological histological subtypes; (D) Differential expression levels of SPOCK1 in patients with or without lymph node metastasis. E Heatmap of the distribution of SPOCK1 and EMT-related markers in the combined cohort and (F) NSCLC cell lines, and the mesenchymal score and epithelial score were assessed based on the ssSGEA algorithm, and correlations were calculated (spearman test). G The potential association between SPOCK1 and dysregulated oncogenic pathways in the combined cohort and (H) NSCLC cell lines. (***p < 0.001; **p < 0.01; *p < 0.05; ns. no significance.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38087364), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Testican 1/SPOCK1 by Flow Cytometry

Detection of Testican 1/SPOCK1 by Flow Cytometry

Role of SPOCK1 in the tumor microenvironment. A Heatmap of the distribution of 29 TME associated signatures with SPOCK1 expression values. B Scatter plot of the correlation between 4 TME scores and SPOCK1 expression values. C Histogram of correlation between SPOCK1 and 5 immune features and chord plot of correlation with TGF-beta 1 (TGFB1), TGF-beta 2 (TGFB2), and TGF-beta 3 (TGFB3) (spearman test). D Distribution of SPOCK1 expression in TME subtypes defined by Bagaev et al. E Correlation between SPOCK1 and CD274 (mRNA level) and PD- L1 (protein level, from TCPA database) correlation scatter plots. (***p < 0.001; **p < 0.01; *p < 0.05; ns. no significance.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38087364), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Testican 1/SPOCK1 by Flow Cytometry

Detection of Testican 1/SPOCK1 by Flow Cytometry

Role of SPOCK1 in the tumor microenvironment. A Heatmap of the distribution of 29 TME associated signatures with SPOCK1 expression values. B Scatter plot of the correlation between 4 TME scores and SPOCK1 expression values. C Histogram of correlation between SPOCK1 and 5 immune features and chord plot of correlation with TGF-beta 1 (TGFB1), TGF-beta 2 (TGFB2), and TGF-beta 3 (TGFB3) (spearman test). D Distribution of SPOCK1 expression in TME subtypes defined by Bagaev et al. E Correlation between SPOCK1 and CD274 (mRNA level) and PD- L1 (protein level, from TCPA database) correlation scatter plots. (***p < 0.001; **p < 0.01; *p < 0.05; ns. no significance.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38087364), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Testican 1/SPOCK1 by Flow Cytometry

Detection of Testican 1/SPOCK1 by Flow Cytometry

Relationship of SPOCK1 with methylation and mutation. Boxplot indicates the extent of variation in promoter methylation levels in LUAD samples from the UALCAN database, (A) in normal and tumor, (B) TP53 mutant and TP53 wild-type groups. C Exploration of the mutational landscape of SPOCK1 in LUAD using the cBioPortal database. Mutation waterfall plots of the top 20 genes in the (D) high SPCOK1 group and (E) low SPCOK1 group in terms of mutation frequency. F Boxplots show the difference of DNA damage measures between different levels of SPOCK1 expression groups (Wilcon rank-sum test). (***p < 0.001; **p < 0.01; *p < 0.05; ns. no significance.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38087364), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Testican 1/SPOCK1 Antibody (316801)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI) under non-reducing conditions

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Testican 1/SPOCK1

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Alternate Names

SPOCK1, TIC1, TICN1

Entrez Gene IDs

6695 (Human)

Gene Symbol

SPOCK1

UniProt

Q08629

Additional Testican 1/SPOCK1 Products

Product Documents for Human Testican 1/SPOCK1 Antibody (316801)

Certificate of Analysis

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Product Specific Notices for Human Testican 1/SPOCK1 Antibody (316801)

For research use only

Citations for Human Testican 1/SPOCK1 Antibody (316801)

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