Human Total ROR1 DuoSet IC ELISA

R&D Systems | Catalog # DYC2000-2

R&D Systems
Discontinued Product
DYC2000-2 has been discontinued. View all ROR1 products.

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

312-20000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human

Human Total ROR1 DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
View all ROR1 ELISA Kits »

Product Summary for Human Total ROR1 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ROR1 in cell lysates. An immobilized capture antibody specific for ROR1 binds both phosphorylated and unphosphorylated ROR1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Total ROR1 DuoSet IC ELISA

Standard Curve

Kit Contents for Human Total ROR1 DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ROR1

ROR1 and ROR2 are receptor tyrosine kinases characterized by an extracellular immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, a kringle domain, and an intracellular kinase domain. RORs have been implicated in cartilage and growth plate development, and neurite outgrowth.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Alternate Names

NTRKR1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Gene Symbol

ROR1

Additional ROR1 Products

Product Documents for Human Total ROR1 DuoSet IC ELISA

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Total ROR1 DuoSet IC ELISA

For research use only

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