Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human TRAF-6
Met1-Val522
Accession # Q9Y4K3
Met1-Val522
Accession # Q9Y4K3
Specificity
Detects human TRAF-6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -4, and -5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TRAF‑6 Antibody
Detection of Human TRAF‑6 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, -TRAF5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-6 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human TRAF‑6 by Simple WesternTM.
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for TRAF‑6 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRAF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human TRAF‑6 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HEK293T human embryonic kidney cell line
Sample: HEK293T human embryonic kidney cell line
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line
Sample: Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 4 using AF3284 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TRAF-6
Long Name
TNF Receptor-Associated Factor 6
Alternate Names
TRAF6
Gene Symbol
TRAF6
UniProt
Additional TRAF-6 Products
Product Documents for Human TRAF‑6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TRAF‑6 Antibody
For research use only
Citations for Human TRAF‑6 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
