Human Transglutaminase 7/TGM7 Antibody

Catalog #: AF5426 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5426
AF5426-SP

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Detection of Human Transglutaminase 7/TGM7 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Transglutaminase 7/TGM7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Transglutaminase 3 is observed, and less than 2% cross-reactivity with rhTransglutaminase 2, rhTransglutaminase 4, and recombinant mouse Transglutaminase 2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Transglutaminase 7/TGM7 by Western Blot. Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 7/TGM7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 7/TGM7 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 7/TGM7

Transglutaminase 7 (TG7), encoded by the TGM7 gene, is also known as protein-glutamine-g-glutamyltransferase Z (Tgase Z) (1). It belongs to the family of Transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (2‑4). TG7 is ubiquitously expressed in humans (1). Members of the TGM family have been implicated in a variety of human diseases including neurodegenerative diseases, celiac disease, lamellar ichthyosis, bleeding disorders, cataract formation, atherosclerosis, and others (5).

References

Grenard, P. et al. (2001) J. Biol. Chem. 276:33066.
Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
Griffin, M. et al. (2002) Biochem. J. 368:377.
Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.

Entrez Gene IDs

116179 (Human); 640543 (Mouse); 691932 (Rat)

Alternate Names

protein-glutamine gamma-glutamyltransferase Z; TG(Z); TGase Z; TGase-7; TGM7; TGMZ; TGMZEC 2.3.2.13; TGZ; Transglutaminase 7; Transglutaminase Z; transglutaminase-7