Human TREM2 Antibody (263602)

Detection of TREM2 by Immunocytochemistry/ Immunofluorescence

TREM2 p.Q33X mutation abolishes cell surface expression of TREM2 receptor on iMGLs and abrogates sTREM2 production. a Diagram representing patient-derived iMGLs from NR CTRL, SB CTRL, NHD1 and NHD2 lines. b and c RT-qPCR of TREM2 and TYROBP mRNA in NR CTRL, SB CTRL, NHD1 and NHD2 iMGLs. ***P < 0.001, ****P < 0.0001, One-way ANOVA with Tukey’s post hoc test. Data are pooled from two independent experiments. d-g FACS analysis of TREM2 receptor expression at iMGL cell surface. d Representative dot plots showing the percentage of TREM2+ cells in the CD45int CD11b+ population. e Representative histogram of TREM2 mean fluorescent intensity (MFI) in SB CTRL, NHD1 and NHD2 iMGLs. f Quantification of TREM2 MFI and g TREM2+ iMGLs in NR CTRL, SB CTRL, NHD1 and NHD2 iMGLs. ****P < 0.0001, One-way ANOVA with Tukey’s post hoc test. Data are pooled from four independent experiments. h Representative confocal images of iMGLs from SB CTRL, NHD1 and NHD2 stained for TMEM119 (green), TREM2 (red) and DAPI (blue). Scale bar, 10 μm. i Media from NR CTRL, SB CTRL, NHD1 and NHD2 iMGLs were collected during iMGLs differentiation and sTREM2 was measured by ELISA. *P < 0.05, Two-way ANOVA with Dunnett’s post hoc test. Data are pooled from two independent experiments. Data shown are mean ± SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37115208), licensed under a CC-BY license. Not internally tested by R&D Systems.