TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK Receptor (TWEAK R, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Arg93-His249
Accession # O43508
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TWEAK/TNFSF12 Antibody (173830)
Human TWEAK / TNFSF12 ELISA Standard Curve
Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) was serially diluted and captured by Mouse Anti-Human TWEAK/TNFSF12 Monoclonal Antibody (Catalog # MAB1090) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human TWEAK/TNFSF12 Antibody (173830)
Human TWEAK/TNFSF12 Sandwich Immunoassay
Reviewed Applications
Read 1 review rated 5 using MAB1090 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TWEAK/TNFSF12
References
- Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
- Marsters, S. et al. (1998) Current Biol. 8:525.
- Wiley, S.R. et al. (2001) Immunity, 15:837.
- Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253.
- Nakayama, M. et al. (2002) J. Immunol. 168:734.
- Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
- Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TWEAK/TNFSF12 Products
Product Documents for Human TWEAK/TNFSF12 Antibody (173830)
Certificate of Analysis
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Product Specific Notices for Human TWEAK/TNFSF12 Antibody (173830)
For research use only
Related Research Areas
Customer Reviews for Human TWEAK/TNFSF12 Antibody (173830) (1)
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/08/2019This antibody was used as the capture in an ELISA along with AF1090 as the detection.
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