Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 393112
View all VAP-1/AOC3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VAP‑1/AOC3
Gly27-Asn763
Accession # Q16853

Specificity

Detects human VAP‑1/AOC3 in direct ELISAs and Western blots. In Western blots, 50% cross-reactivity with recombinant mouseVAP‑1/AOC3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VAP‑1/AOC3 Antibody

Detection of VAP-1/AOC3 by Western Blot

Detection of VAP-1/AOC3 by Western Blot

Biophysical and functional characterization of endometrial pericytes and EnSCs. (A) Immunohistochemistry showing VAP-1 expression in luteal-phase human endometrium, hematoxylin was used to stain the nuclei. Staining indicates VAP-1 reactivity in the vasculature and spiral arteries, arrow pointing to spiral artery. Scale bar: 200 μm. (B) VAP-1 expression was determined at protein level in freshly isolated SUSD2+ pericytes and SUSD2− EnSCs from three independent endometrial biopsies by Western blot analysis and normalized to beta -actin and relative intensity determined by densitometry; Student's t-test; *P < 0.05. (C) Expression of ELN (left panel), CNN1 (middle panel) and MYH11 (right panel) transcripts was determined by RT-qPCR in freshly isolated SUSD2+ pericytes and SUSD2− EnSCs from four independent endometrial biopsies; Student's t-test; *P < 0.05; **P < 0.01. (D) Representative graphs showing proliferation and migration SUSD2+ pericytes and SUSD2− EnSCs monitored in real-time using the xCELLigence system for the indicated time-points. (E) freshly isolated SUSD2+ pericytes and SUSD2− EnSCs were embedded into collagen at a density of 1 × 106 cells per gel. Single cell contraction force was measured using the depth-sensing nanoindentation system. Data represent mean ± SEM of four biological repeat experiments. Student's t-test; **P < 0.01; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33537312), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VAP‑1/AOC3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human VAP‑1/AOC3 (Catalog # 3957-AO) under reducing conditions only

Reviewed Applications

Read 1 review rated 3 using MAB3957 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAP-1/AOC3

Vascular adhesion protein-1 (VAP-1), also called AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase), is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). Human and mouse VAP-1 share 83% amino acid sequence identity. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2 (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).

References

  1. Kurkijärvi, R. et al. (1998) J. Immunol. 161:1549.
  2. Gearing, A.J.H. and W. Newman (1993) Immunol. Today 14:506.
  3. Lizcano, J.M. et al. (1998) Biochem. J. 331:69.
  4. Smith, D.J. et al. (1998) J. Exp. Med. 188:17.
  5. Jaakkala K. et al. (2000) Am. J. Pathol. 157:463.
  6. Salmi, M. and J. Jalkanen (2001) Trends Immunol. 22:211.
  7. Salmi, M. and J. Jalkanen (1996) J. Exp. Med. 183:569.
  8. Dunkel, P. et al. (2008) Curr. Med. Chem. 15:1827.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Entrez Gene IDs

8639 (Human); 11754 (Mouse); 29473 (Rat)

Gene Symbol

AOC3

UniProt

Q16853

Additional VAP-1/AOC3 Products

Product Documents for Human VAP‑1/AOC3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VAP‑1/AOC3 Antibody

For research use only

Citations for Human VAP‑1/AOC3 Antibody

Customer Reviews for Human VAP‑1/AOC3 Antibody (1)

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  • Human VAP-1/AOC3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Endometrium
    Species: Equine
    Verified Customer | Posted 02/20/2020
    The Antibody worked great in a Positive control (Human Lung) but no reactivity to the equine protein was possible.
    Human VAP‑1/AOC3 Antibody MAB3957

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