Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 122627
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF-B186
aa 22-207
Accession # P49765

Specificity

Detects human VEGF-B in direct ELISAs and Western blots. Recognizes both the 167 and the 186 amino acid isoforms of human VEGF-B. In direct ELISAs, this antibody does not cross-react with any isoforms of recombinant human (rh) VEGF or rmVEGF-B or with rhVEGF-C, rhVEGF-D, rhCTGF, rhLDGF, or rrVEGF164.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human VEGF-B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human VEGF-B167 (Catalog # 751-VE)

Reviewed Applications

Read 1 review rated 5 using MAB3372 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.

Long Name

Vascular Endothelial Growth Factor B

Alternate Names

VEGFB

Entrez Gene IDs

7423 (Human); 22339 (Mouse)

Gene Symbol

VEGFB

UniProt

Additional VEGF-B Products

Product Documents for Human VEGF-B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGF-B Antibody

For research use only

Citations for Human VEGF-B Antibody

Customer Reviews for Human VEGF-B Antibody (1)

5 out of 5
1 Customer Rating
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  • Human VEGF-B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/18/2023
    https://www.frontiersin.org/articles/10.3389/fbioe.2022.907538/full
    Human VEGF-B Antibody MAB3372

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