Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 122627
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF-B186
aa 22-207
Accession # P49765
aa 22-207
Accession # P49765
Specificity
Detects human VEGF-B in direct ELISAs and Western blots. Recognizes both the 167 and the 186 amino acid isoforms of human VEGF-B. In direct ELISAs, this antibody does not cross-react with any isoforms of recombinant human (rh) VEGF or rmVEGF-B or with rhVEGF-C, rhVEGF-D, rhCTGF, rhLDGF, or rrVEGF164.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human VEGF-B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human VEGF-B167 (Catalog # 751-VE)
Sample: Recombinant Human VEGF-B167 (Catalog # 751-VE)
Reviewed Applications
Read 1 review rated 5 using MAB3372 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: VEGF-B
Long Name
Vascular Endothelial Growth Factor B
Alternate Names
VEGFB
Gene Symbol
VEGFB
UniProt
Additional VEGF-B Products
Product Documents for Human VEGF-B Antibody
Product Specific Notices for Human VEGF-B Antibody
For research use only
Citations for Human VEGF-B Antibody
Customer Reviews for Human VEGF-B Antibody (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: HEK293T human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 04/18/2023https://www.frontiersin.org/articles/10.3389/fbioe.2022.907538/full
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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