Human VISTA/B7-H5/PD-1H Antibody (3180B)

R&D Systems | Catalog # MAB11704

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3180B
View all VISTA/B7-H5/PD-1H Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q9H7M9

Specificity

Detects a synthetic peptide specific for human VISTA around amino acid 100 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human VISTA/B7-H5/PD-1H Antibody (3180B)

Detection of Human VISTA/B7-H5/PD-1H by Western Blot.

Western Blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human VISTA/B7-H5/PD-1H by Simple WesternTM.

Left: Simple Western lane view shows lysates of human placenta, loaded at 0.1 mg/ml. A specific band was detected for VISTA/B7-H5/PD-1H at approximately 59 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in human placenta.

Applications for Human VISTA/B7-H5/PD-1H Antibody (3180B)

Application
Recommended Usage

Simple Western

2-10 µg/mL
Sample: Human placenta

Western Blot

2 µg/mL
Sample: Human placenta

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

Q9H7M9 (Human)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Human VISTA/B7-H5/PD-1H Antibody (3180B)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VISTA/B7-H5/PD-1H Antibody (3180B)

For research use only

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