Human Vitamin D BP Antibody (359501)

R&D Systems | Catalog # MAB3778

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 359501
View all Vitamin D BP Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived Vitamin D BP
Accession # P02774

Specificity

Detects human plasma-derived Vitamin D BP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Vitamin D BP Antibody (359501)

Detection of Vitamin D BP by Western Blot

Detection of Vitamin D BP by Western Blot

Legumain is required for VDBP processing and regulation. (A) Purified VDBP from human plasma (1.9 μM) was incubated in legumain assay buffer (pH 5.8) at 37 °C with or without purified active bovine legumain (2 μM) for 5 h before gel electrophoresis and immunoblotting of VDBP (n = 1). (B–H) Wild-type (Lgmn+/+) and legumain-deficient (Lgmn−/−) mice were treated with 50 µg/kg 25(OH)D3 (n = 6–7) or an equal volume vehicle (n = 7, control) subcutaneously every two to three days (four times in total). Tissues were harvested 24 h after the final injection (day 8). (B) One representative immunoblot of VDBP and GAPDH (housekeeping) in kidney and liver (n = 4). (C–F) Quantification of VDBP immunoband (IB) intensity as arbitrary units (ARBU) relative to GAPDH in immunoblots represented in (B) (n = 4). (C) Hepatic VDBP 45 kDa immunoband. (D) Renal VDBP 45 kDa immunoband. (E) Hepatic VDBP 55 kDa immunoband. (F) Renal VDBP 55 kDa immunoband. (G) Plasma VDBP concentration (μg/mL) was measured by ELISA (n = 6–7). (H) Hepatic VDBP mRNA expression relative to the geometric mean of CT values of four housekeeping controls (2− delta delta CT, n = 5). (C–H) Data represent mean ± SEM. Two-way ANOVA. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. different genotype, same treatment. Numbers (n) represent individual biological replicates. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/13/1/36), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Vitamin D BP Antibody (359501)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human plasma-derived Vitamin D BP

Reviewed Applications

Read 1 review rated 5 using MAB3778 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vitamin D BP

Vitamin D BP (VitD BP), also called VDB, DBP, Group Specific Component (Gc) or macrophage-activating factor (DBP- or Gc-MAF), is a member of the albumin family. These plasma/body fluid proteins have a conserved pattern of cysteine motifs that form binding domains. VitD BP is predominantly produced by hepatic parenchymal cells. It transports VitD, activates macrophages and osteoclasts, and enhances C5a chemotactic activity. Over 80 variant alleles have been identified, some of which correlate with osteoporosis or prostate cancer. Human and mouse VitD BP share 78% aa identity.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Entrez Gene IDs

2638 (Human); 14473 (Mouse); 24384 (Rat)

Gene Symbol

GC

UniProt

P02774

Additional Vitamin D BP Products

Product Documents for Human Vitamin D BP Antibody (359501)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Vitamin D BP Antibody (359501)

For research use only

Citations for Human Vitamin D BP Antibody (359501)

Customer Reviews for Human Vitamin D BP Antibody (359501) (1)

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  • Human Vitamin D BP Antibody
    Name: Tatjana Bosnjak
    Application: Immunohistochemistry-Frozen
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 09/09/2019
    VDBP (red) in mouse renal tissue.
    MAB3778 concentration used was 1 µg/ml. Secondary antibody was Alexa Fluor conjugated goat anti-mouse 594 (red; 1:1000).
    Human Vitamin D BP Antibody (359501) MAB3778

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