Human WARP Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB6189S Datasheet / COA / SDS

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FAB6189S-100UG

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Human WARP Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human WARP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) WARP, rmCollagen II, or recombinant bovine Collagen I is observed.

Source

Monoclonal Mouse IgG1 Clone # 559915

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WARP
Arg19-Pro445
Accession # Q6PCB0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WARP

Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50 kDa glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa 34‑213), two fibronectin type III domains (aa 211‑301 and 331‑421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP shares 72% aa sequence identity with mature mouse and rat WARP.

Long Name

von Willebrand factor A Domain-related Protein

Entrez Gene IDs

64856 (Human); 246228 (Mouse); 298683 (Rat)

Alternate Names

DKFZp761O051; von Willebrand factor A domain containing 1; von Willebrand factor A domain-containing protein 1; von Willebrand factor A domain-related protein; VWA1; WARP