iASPP Antibody

Novus Biologicals | Catalog # NB100-88155

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all iASPP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human inhibitor of apoptosis stimulating protein of p53 using the numbering given in entry NP_006654.2 (GeneID 10848).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for iASPP Antibody

Western Blot: iASPP Antibody [NB100-88155]

Western Blot: iASPP Antibody [NB100-88155]

Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: iASPP Antibody [NB100-88155]

Immunoprecipitation: iASPP Antibody [NB100-88155]

Immunoprecipitation: iASPP Antibody [NB100-88155] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiiASPP antibody used for IP at 6 ug per reaction. iASPP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated iASPP, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes

Applications for iASPP Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: iASPP

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM]

Alternate Names

IASPPinhibitor of apoptosis stimulating protein of p53, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13BL, PPP1R13B-like protein, Protein iASPP, protein phosphatase 1, regulatory (inhibitor) subunit 13 like, protein phosphatase 1, regulatory subunit 13 like, RAINFkB interacting protein 1, relA-associated inhibitor

Entrez Gene IDs

10848 (Human)

Gene Symbol

PPP1R13L

UniProt

Q8WUF5 (Human)

Additional iASPP Products

Product Documents for iASPP Antibody

Certificate of Analysis

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Product Specific Notices for iASPP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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