IFT74 Antibody

Novus Biologicals | Catalog # NB300-822

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IFT74 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KTIVDALHSTSGN corresponding to C-Terminus according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.

Reactivity Notes

Expected species reactivity based on sequence similarity: Rat

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IFT74 Antibody

Western Blot: IFT74 Antibody [NB300-822]

Western Blot: IFT74 Antibody [NB300-822]

Western Blot: IFT74 Antibody [NB300-822] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antinbody at 0.2 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IFT74 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2 - 0.6 ug/mL
Application Notes
IF successful usage for stained rat cortical neurons is reported in scientific literature (PMID: 17166276). WB: Approx. 70-75 kDa band observed in HEK293 lysates is reported in scientific literature (PMID: 17166276).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFT74

The IFT74 gene codes for an intraflagellar transport protein 74 homolog. Isoform 1 is 600 amino acids long at 69 kDA while isoform 2 exists at 409 amino acids long at 46 kDA. IFT74 has been linked to dementia, lateral sclerosis, neuronitis, amyotrphic lateral sclerosis, and frontotemporal dementia. It is known to interact with other genes such as: IFT27, IFT52, IFT81, IFT88, and DYNC2LI1.

Alternate Names

Capillary morphogenesis gene 1 protein, CCDC2capillary morphogenesis protein 1, CMG1Coiled-coil domain-containing protein 2, CMG-1MGC111562, coiled-coil domain containing 2, FLJ22621, intraflagellar transport 74 homolog (Chlamydomonas), intraflagellar transport protein 74 homolog

Entrez Gene IDs

80173 (Human); 313365 (Rat)

Gene Symbol

IFT74

UniProt

NP_001092692.1 (Human), NP_001092693.1 (Human), NP_079379.2 (Human), AAK77221.1 (Human)

Additional IFT74 Products

Product Documents for IFT74 Antibody

Certificate of Analysis

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Product Specific Notices for IFT74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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