IgE Antibody (RM122) [Biotin]

Novus Biologicals | Catalog # NBP2-62027B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM122 expressed in HEK293
View all IgE Primary Antibodies »

Product Specifications

Immunogen

Human IgE

Specificity

This antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgE Antibody (RM122) [Biotin]

IgE Antibody (RM122) [Biotin]

ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP2-62027B] -

ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP2-62027B] - A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgE Antibody (RM122) [Biotin]

ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP2-62027B] -

ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP2-62027B] - ELISA of human immunoglobulins shows RM122 reacts to both IgE from human myeloma and IgE from human plasma. No cross reactivity with human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgE Antibody (RM122) [Biotin]

Application
Recommended Usage

Sandwich ELISA Capture

100 ng/well

Sandwich ELISA Detection

0.01 - 0.1 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgE

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for IgE Antibody (RM122) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IgE Antibody (RM122) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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