IgG Antibody (RM104)

Novus Biologicals | Catalog # NBP2-62005

Recombinant Monoclonal Antibody.
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM104 expressed in HEK293
View all IgG Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IgG Antibody (RM104) (NBP2-62005) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IgG Antibody (RM104)

Western Blot: IgG Antibody (RM104) [NBP2-62005] -

Western Blot: IgG Antibody (RM104) [NBP2-62005] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20 ng/lane), using NBP2-62005 at 0.2ug/ml. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
IgG Antibody (RM104)

ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] -

ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] - ELISA of mouse immunoglobulins shows RM104 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG Antibody (RM104)

ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] -

ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG Antibody (RM104)

Application
Recommended Usage

ELISA

0.005 ug/ml-0.2 ug/ml

Western Blot

0.1 ug/ml-0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for IgG Antibody (RM104)

Certificate of Analysis

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Product Specific Notices for IgG Antibody (RM104)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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