Equine IgG ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60067

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.6 ug/mL

Assay Range

0.6 ug/mL - 300 ug/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Equine

Specificity

This assay has high sensitivity and excellent specificity for detection of horse IgG. No significant cross-reactivity or interference between horse IgG and analogues was observed.

Description

Assay Length: 1-5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Equine IgG ELISA Kit (Colorimetric)

Equine IgG ELISA Kit (Colorimetric)

Equine IgG ELISA Kit (Colorimetric) [NBP2-60067] -

Equine IgG ELISA Kit (Colorimetric) [NBP2-60067] - Standard Curve Reference

Kit Contents for Equine IgG ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rat IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for rat IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of rat IgG in samples. The color development is stopped and the intensity of the color is measured.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Equine IgG ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Equine IgG ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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