IgG1 Antibody (RM106) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25950

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM106 expressed in HEK293

Format

Azide and BSA Free
View all IgG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG1 Antibody (RM106) - Azide and BSA Free

IgG1 Antibody (RM106) - Azide and BSA Free

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] -

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] - A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG1 Antibody (RM106) - Azide and BSA Free

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] -

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] - ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG1 Antibody (RM106) - Azide and BSA Free

Western Blot: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] -

Western Blot: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using Clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced g1 form (~50 kDa).
IgG1 Antibody (RM106) - Azide and BSA Free

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] -

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] - ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG1 Antibody (RM106) - Azide and BSA Free

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] -

ELISA: IgG1 Antibody (RM106) - Azide and BSA Free [NBP3-25950] - A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG1 Antibody (RM106) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.005 ug/ml-0.2 ug/ml

Western Blot

0.1 ug/ml-0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for IgG1 Antibody (RM106) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IgG1 Antibody (RM106) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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