INF2 Antibody

Novus Biologicals | Catalog # NBP1-79027

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all INF2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1050 and 1100 of human Inverted Formin-2 using the numbering given in entry NP_071934.3 (GeneID 64423).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for INF2 Antibody

Western Blot: INF2 Antibody [NBP1-79027]

Western Blot: INF2 Antibody [NBP1-79027]

Western Blot: INF2 Antibody [NBP1-79027] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-INF2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. INF2 was also immunoprecipitated by rabbit anti-INF2 antibodies NBP1-79025 and NBP1-79026 which recognize other epitopes.

Applications for INF2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10ug/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: INF2

Actin filaments grow only when actin monomers have access to the fast-growing barbed end of the filament. The geometry of the filament network depends on the actions of the ARP2/3 complex (MIM 604221) and members of the formin family, such as INF2. The ARP2/3 complex binds to the sides of preexisting filaments and nucleates filaments whose barbed ends are quickly blocked by capping proteins, producing brush-like structures, such as those found at the leading edges of crawling cells. In contrast, formins bind to the barbed ends of growing filaments and protect them from capping, creating long filaments that can be cross-linked into bundles, such as those found in actin cables of yeast. Interaction of formins with actin barbed ends occurs through the formin homology-2 (FH2) domain. FH2 domains accelerate filament nucleation, move with the barbed end as the filament grows, and block capping of the barbed end by proteins such as gelsolin (GSN; MIM 137350). The FH1 domain of formins binds to profilin (see MIM 176610) and accelerates elongation from the FH2-bound barbed ends (Bindschadler and McGrath, 2004 [PubMed 15466701]; Chhabra and Higgs, 2006 [PubMed 16818491]).[supplied by OMIM]

Alternate Names

C14orf151, C14orf173DKFZp762A0214, chromosome 14 open reading frame 151, chromosome 14 open reading frame 173, FLJ22056, FSGS5, HBEAG-binding protein 2 binding protein C, HBEBP2-binding protein C, inverted formin, FH2 and WH2 domain containing, inverted formin-2, MGC13251, pp9484

Gene Symbol

INF2

UniProt

NP_071934.3 (Human)

Additional INF2 Products

Product Documents for INF2 Antibody

Certificate of Analysis

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Product Specific Notices for INF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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