Recombinant Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006) His Protein

Novus Biologicals | Catalog # NBP1-99037

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Key Product Details

Source

Baculovirus

Tag

His

Applications

ELISA, SDS-PAGE
View all Influenza A H3N2 Hemagglutinin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-344 of (Canine) Influenza A H3N2

Source: Baculovirus Amino Acid Sequence: ADPNLPGNENN AATLCLGHHA VPNGTIVKTI TDDQIEVTNA TELVQNSSTG KICNNPHKIL DGRDCTLIDA LLGDPHCDVF QNETWDLFVE RSNAFSNCYP YDVPDYASLR SIVASSGTLE FITEGFTWAG VTQNGGSGAC KKGPANGFFS RLNWLTKSGN TYPVLNVTMP NNNNFDKLYI WGVHHPSTNQ EQTSLYIQAS GRVKVSTRRS QQTIIPNIGS RPLVRGQSGR ISVYWTIVKP GDVLVINSNG NLIAPRGYFK MRIGKSSIMR SDAPIDTCIS ECITPNGSIP NEKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPERQTH HHHHH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in ELISA reported in secitific publication PMID: 32849530

Specificity

Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006) His Protein [NBP1-99037]

SDS-PAGE: Recombinant Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006) His Protein [NBP1-99037]

SDS-Page: Recombinant Influenza A Virus H3N2 Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006) His Protein [NBP1-99037] - SDS-Page: Recombinant Virus Influenza A H3N2 Hemagglutinin Protein [NBP1-99037]

Formulation, Preparation, and Storage

NBP1-99037
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H3N2 Hemagglutinin

Influenza A virus subtype H3N2, also known as H3N2, is a subtype of viruses that cause influenza (flu). H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. Hemagglutinin(HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Additional Influenza A H3N2 Hemagglutinin Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Influenza A H3N2 Hemagglutinin (A/canine/Guangdong/1/2006) His Protein

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FAQs

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  • Q: Does the protein NBP1-99037 have infectivity?

    A: No, NBP1-99037 is not infectious. The purpose of manufacturing this product by our lab is for using as the positive control in WB/PAGE.  NBP1-99037 is a recombinant protein, containing mainly the amino acids 18-344 derived from the hemagglutinin (HA) of the Influenza A H3N2 (canine) strain.  Although HA has been know to bind to the receptor on the cell surface, the carbohydrate moiety is responsible for interaction.  Fortunately, the E. coli-expressed proteins won't have the glycosylated modifications, hence this protein is not going to bind to influenza's receptor. In summary, this is just a regular recombinant protein, similar to many others, that do not post any harmful effect of any kind to human.

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