ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

Novus Biologicals | Catalog # NBP3-33446

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6V3Y5
View all ISG15 Activating Enzyme/UBE1L Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human ISG15 Activating Enzyme/UBE1L (NP_003326.2).

Sequence:
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5) [NBP3-33446] -

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5) [NBP3-33446] - Western blot analysis of lysates from HEL cells, using ISG15 Activating Enzyme/UBE1L Rabbit mAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ISG15 Activating Enzyme/UBE1L

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.

Alternate Names

D8, UBA1B, UBA7, UBE2

Gene Symbol

UBA7

Additional ISG15 Activating Enzyme/UBE1L Products

Product Documents for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ISG15 Activating Enzyme/UBE1L Antibody (6V3Y5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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