Janelia Fluor® 646, NHS ester

Tocris Bioscience | Catalog # 6148

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Alternative Names

JF 646,NHS

Wavelength

Excitation = 646 nm, Emission = 664 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 646, NHS ester is a red fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 549 NHS ester (Cat. No. 6147). Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in TFE plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We recommend that stock solutions of this dye are prepared in anhydrous DMF.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 646, SE.

Scientific Data Images for Janelia Fluor® 646, NHS ester

Super-resolution imaging of methylated histones (H3K9me3) in a COS-7 cell line

Super-resolution imaging of methylated histones (H3K9me3) in a COS-7 cell line.

COS-7 cells were fixed and stained with rabbit anti-histone H3K9me3 (Active Motif, Catalog # 39062, 1:1000 dilution) followed by Janelia Fluor® 646 labelled anti-rabbit IgG (H+L) secondary antibody (Novus, Catalog # NBP1-75335, 1:2000 dilution). Super-resolution images were acquired with the Sysmex HM-1000. Images were acquired as 20000 frame stack files with 30 msec. exposure time and image was constructed using HM-1000 software or ThunderSTORM.
Application of Janelia Fluor® 646, NHS ester in Cardiac Tissue.

Application of Janelia Fluor® 646, NHS ester in Cardiac Tissue.

Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. Janelia Fluor 646, NHS ester was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale: 2 μm.Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 646, NHS ester Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.19
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Trademarks and registered trademarks are the property of their respective owners.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for Janelia Fluor® 646, NHS ester

Molecular Weight

593.71

Formula

C33H31N3O6Si

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

CAS Number

1811539-59-9

PubChem ID

124201858

InChI Key

ODBCGJUBBYOJOS-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 29.69 50
DMSO 2.97 5

Preparing Stock Solutions for Janelia Fluor® 646, NHS ester

The following data is based on the product molecular weight 593.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.37 mL 16.84 mL 33.69 mL
2.5 mM 0.67 mL 3.37 mL 6.74 mL
5 mM 0.34 mL 1.68 mL 3.37 mL
25 mM 0.07 mL 0.34 mL 0.67 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Product Documents for Janelia Fluor® 646, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 646, NHS ester

For research use only

Citations for Janelia Fluor® 646, NHS ester

Customer Reviews for Janelia Fluor® 646, NHS ester (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Janelia Fluor® 646, NHS ester?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • JF646 labeled proteins in vitro
    Name: Saumya Saurabh
    Species: Bacteria
    Assay Type: In Vitro
    Verified Customer | Posted 02/26/2018
    I used this dye for labeling bacterial proteins in vitro. The labeling method is pretty straight-forward as for any NHS-protein coupling. The dye is highly photostable as determined by single protein tracking on lipid bilayers (image of single proteins attached, scale bar is 5 micron). The only reason I give the product 4 stars instead of 5 is the packaging. The dye is shipped as a solid in a glass bottle with a flexible rubber cap. Most of the dye gets stuck inside and on the surface of this cap; thus not delivering a full value for money. I will highly recommend the manufacturers to use bottles with plastic caps so that the dye can be recovered a 100%
    Janelia Fluor® 646, NHS ester 6148

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All