KF1 Antibody (3E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00007844-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E7

Format

Azide and BSA Free
View all KF1 Primary Antibodies »

Product Specifications

Immunogen

RNF103 (NP_005658, 501 a.a. ~ 599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYN

Specificity

RNF103 - ring finger protein 103

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KF1 Antibody (3E7) - Azide and BSA Free

Western Blot: KF1 Antibody (3E7) [H00007844-M01]

Western Blot: KF1 Antibody (3E7) [H00007844-M01]

Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi ( Cat # H00007844-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 (Cat # H00007844-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: KF1 Antibody (3E7) [H00007844-M01]

Western Blot: KF1 Antibody (3E7) [H00007844-M01]

Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

Applications for KF1 Antibody (3E7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KF1

The protein encoded by this gene contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants.

Alternate Names

E3 ubiquitin-protein ligase RNF103, EC 6.3.2.-, hKF-1, KF-1, KF1ZFP103ZFP-103, MGC102815, ring finger protein 103MGC41857, Zfp-103, Zinc finger protein 103 homolog, zinc finger protein 103 homolog (mouse), zinc finger protein expressed in cerebellum

Entrez Gene IDs

7844 (Human)

Gene Symbol

RNF103

OMIM

602507 (Human)

UniProt

NP_005658 (Human)

Additional KF1 Products

Product Documents for KF1 Antibody (3E7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KF1 Antibody (3E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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