Key Product Details

Species Reactivity

Bacteria

Applications

ELISA

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # 2F10
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Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with membrane fraction of Legionella pneumophila, strain Philadelphia 1.

Reactivity Notes

Bacteria

Specificity

Reacts with LPS membrane of Legionella pneumophila. No cross-reactivity with Legionella pneumophila serogroups 2, 6, 7, 10 and 11, Legionella bozemanii, Legionella lonbeachae, Legionella micdadei, Corynebacterium diphtheria, Pseudomonas fluorescens, Pseudomonas cepacia, Pseudomonas aerugenose, Bordetella pertusis, Leptospira interrogens (Australia, Pomona, Icterogemorrhagia), Toxoplasma gondii, Hemophilus influenza (type B), Hemophilus ducreyi, Brucella abortus, Bacillus anthracis, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium tuberculosis, Neisseria meningitides, Pasteurella multocida, Proteus vulgaris, Shigella sonnei, Staphylococcus faecalis, Streptococcus puogenes, Streptococcus pneumoniae, Yersinia pseudotuberculosis, Yersinia pestis EV76, Salmonella typhi, Escherichia coli K88 and Francisella tularensis 15. Cross-reactivity with Legionella pneumophila serogroups 3 and 4 has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Legionella Pneumophila Antibody (2F10) [DyLight 488]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB110-2520. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Legionella Pneumophila

Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

Alternate Names

L. pneumophila, Legionellosis

Additional Legionella Pneumophila Products

Product Documents for Legionella Pneumophila Antibody (2F10) [DyLight 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Legionella Pneumophila Antibody (2F10) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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