LMAN2 Antibody (02) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05830

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 02

Format

Azide and BSA Free
View all LMAN2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 (Uniprot#: Q12907; Asp 45-Arg 322).

Reactivity Notes

No cross-reactivity in ELISA with:

E.coli cell lysate

Specificity

No cross-reactivity in ELISA with: E.coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LMAN2 Antibody (02) - Azide and BSA Free

LMAN2 Antibody (02) - Azide and BSA Free

Western Blot: LMAN2 Antibody (02) [NBP3-05830] -

Western Blot: LMAN2 Antibody (02) [NBP3-05830] - NBP3-05830 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.)
LMAN2 Antibody (02) - Azide and BSA Free

Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830] -

Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830] - LMAN2-His was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 2 uL NBP3-05830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05830, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 40 kDa Observed band size: 40 kDa

Applications for LMAN2 Antibody (02) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000
Application Notes
This antibody will detect Human LMAN2 in ELISA pair set (Catalog: # NBP2-79398). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP3-05831).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMAN2

The LMAN2 gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quali

Alternate Names

C5orf8, chromosome 5 open reading frame 8, Glycoprotein GP36b, GP36B, Lectin mannose-binding 2, lectin, mannose-binding 2, vesicular integral protein of 36 kDa, vesicular integral-membrane protein VIP36, VIP36

Gene Symbol

LMAN2

Additional LMAN2 Products

Product Documents for LMAN2 Antibody (02) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LMAN2 Antibody (02) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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