Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60084

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Monkey

Specificity

This assay has high sensitivity and excellent specificity for detection of monkey LH. No significant cross-reactivity or interference between monkey LH and analogues was observed.

Description

Assay Length: 1-5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

Monkey Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60084] -

Monkey Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60084] - Standard Curve Reference

Kit Contents for Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

  • Adhesive Strip (for 96-well plate) - 4 EA
  • Antibody - 6 mL
  • Assay Plate - 1 EA
  • HRP-Conjugate - 11 mL
  • Instruction manual
  • Standard - 6 x 0.5 mL
  • Stop Solution - 7 mL
  • Substrate A - 7 mL
  • Substrate B - 7 mL
  • Wash Buffer (20X concentrate) - 15 mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Luteinizing Hormone

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for LH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

CGB4;HH23;LHB;LSH-B;LSH-beta;Lutropin subunit beta

Gene Symbol

LHB

Additional Luteinizing Hormone Products

Product Documents for Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Monkey Luteinizing Hormone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  • No detection in normally-cycling monkeys or positive QCs
    Name: Anonymous
    Sample Tested: Serum
    Species: Rhesus Macaque
    Verified Customer | Posted 07/13/2026
    Results from test run which included a successful curve but no values in samples (including LH surge)
    Kit was run per protocol (by an operator with 17 years' experience running probably thousands of ELISAs by now). To test if the kit can measure LH in normally-cycling monkeys as advertised, we tested sets of serum samples from four different animals including the LH surge and negative controls, plus known-positive QCs. At this point, we'd find a product useful that could distinguish an LH surge from a non-surge sample, but this kit cannot even do that. If it works at all, it is not even close to sensitive enough to measure normal values in monkeys.
    Monkey Luteinizing Hormone ELISA Kit (Colorimetric) NBP2-60084
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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