MagCellect Mouse Naive CD8+ T Cell Isolation Kit

R&D Systems | Catalog # MAGM207

R&D Systems
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Key Product Details

Species

Mouse

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD8

CD8, also known as Ly-2 or Leu-2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes. CD8 alpha is expressed on double positive (CD4+8+) thymocytes and mature CD8+ cytolytic T cells, intraepithelial lymphocytes, some gamma-delta T cells, subsets of thymic and splenic dendritic cells, and peritoneal mast cells. It can form disulfide linked homodimers or heterodimers with CD8 beta. Thymic CD8+ DC express primarily alpha-beta heterodimers, while splenic CD8+ DC primarily express alpha-alpha homodimers. CD8 alpha+ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

For research use only

Citations for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

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    Name: Leslie Priddy
    Verified Customer | Posted 04/20/2018

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FAQs for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

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    • Q: When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
      A: It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.
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