MAP3K12 Antibody

Novus Biologicals | Catalog # NBP1-46063

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MAP3K12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to unique epitope on MAP3K12. The peptide sequence was selected from mid-region, amimo acids 270-320. This peptide was covalently modified post-synthetically to achieve desired antigenicity.

Specificity

Specifically detects a unique epitope on MAP3K12.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP1-46063]

Western Blot: MAP3K12 Antibody [NBP1-46063]

Western Blot: MAP3K12 Antibody [NBP1-46063] - Analysis using the FITC conjugate of NBP1-46063. Detection of MAP3K12 (MAP3K12-AP) antibody (1:500) in diluObuffer with PC-MAP3K12 samples. Ln 1: Mock transfected cells; Ln 2: MAP3K12 transfectred cells.

Applications for MAP3K12 Antibody

Application
Recommended Usage

ELISA

1:1000

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAP3K12

Mitogen-activated protein kinase kinase kinase 12 (MAP3K12) is a MAP kinase kinase kinase enzyme belonging to the serine/threonine protein kinase family. Located on human chromosome 12q13, it regulates the activity of various MAPKKs such as MAPKK7 and hence activating C-Jun N-terminal Kinase (JNK/SAPK). MAP3K12 associates with scaffold proteins, JIPs, which also interacts with MAPKK7 and JNK/SAPK. MAP3K12 protein is associated with dotted structures that are regularly located with golgi apparatus and also along the microtubules. This protein contains two leucine-zipper-like motifs and interaction of MAP3K12-binding inhibitory protein with one of these motifs deactivates this protein and induces activation of SAPK/JNK. MAP3K12 is also implicated in regulating radial cell migration via microtubule-based events. It has been reported that ectopic expression of this protein in neuronal precursor cells allows these cells to migrate from the ventricular zone and differentiate into neural cells. MAP3K12 was identified as a signaling molecule that is required for the regulation of keratinocyte terminal differentiation and cornification. While MAP3K12 is found in the epidermis, it is highly restricted to the neuronal cells in the central, peripheral and autonomic nervous systems. More specifically, it is localized in the axons of these cells. It has been shown that over-expression of MAP3K12 in cos-1 cells impairs the radial organization of microtubules without massive depolymerization. In humans, MAP3K12 is approximately a 68kDa protein (567 amino acids). In rat and mouse this protein weighs 107kDa (888 amino acids).

Alternate Names

DLKEC 2.7.11.25, Dual leucine zipper bearing kinase, EC 2.7.11, Leucine-zipper protein kinase, MAPK-upstream kinase, MEKK12, mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase, MUKdual leucine zipper kinase DLK, protein kinase MUK, ZPKleucine zipper protein kinase, ZPKP1

Entrez Gene IDs

7786 (Human)

Gene Symbol

MAP3K12

UniProt

Q12852 (Human)

Additional MAP3K12 Products

Product Documents for MAP3K12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MAP3K12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAP3K12 Antibody

Customer Reviews for MAP3K12 Antibody

There are currently no reviews for this product. Be the first to review MAP3K12 Antibody and earn rewards!

Have you used MAP3K12 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies