Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 350 and 400 of human MAP/Microtubule Affinity-Regulating Kinase 2 using the numbering given in entry NP_001034558.2 (GeneID 2011).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31018989).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MARK2 Antibody - BSA Free

Western Blot: MARK2 Antibody [NBP1-71890]

Western Blot: MARK2 Antibody [NBP1-71890]

Western Blot: MARK2 Antibody [NBP1-71890] - Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-MARK2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MARK2 was also immunoprecipitated by rabbit anti-MARK2 antibody NBP1-71889, which recognizes an upstream epitope.

Applications for MARK2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MARK2

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.

Alternate Names

EC 2.7.11, EC 2.7.11.1, ELKL motif kinase, ELKL motif kinase 1, EMK-1, EMK1PAR-1, MAP/microtubule affinity-regulating kinase 2MGC99619, PAR1 homolog, Par1b, Ser/Thr protein kinase PAR-1B, serine/threonine protein kinase EMK, serine/threonine-protein kinase MARK2

Gene Symbol

MARK2

UniProt

NP_001034558.2 (Human)

Additional MARK2 Products

Product Documents for MARK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MARK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MARK2 Antibody - BSA Free

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