MD13

Key Product Details

Product Description

MD13 is a potent macrophage migration inhibitory factor (MIF) Degrader (PROTAC^®; DC₅₀ < 100 nM; D_max = 92% after 6 h). MD13 comprises the cereblon ligand Pomalidomide (Cat. No. 6302) joined by a linker to a MIF inhibitor. MD13 reduces MIF levels, arrests cells at the G₂/M phase and inhibits proliferation of A549 lung carcinoma cells. Growth of A549 cell 3D spheroids is also inhibited by MD13.

Negative control MD15 (Cat. No. 7504) and MIF antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF-289-PB and MAB289.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold with kind permission of Professor Dekker.

Product Specifications for MD13

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for MD13

The following data is based on the product molecular weight 653.69.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.53 mL	7.65 mL	15.30 mL
5 mM	0.31 mL	1.53 mL	3.06 mL
10 mM	0.15 mL	0.76 mL	1.53 mL
50 mM	0.03 mL	0.15 mL	0.31 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Xiao Proteolysis targeting chimera (PROTAC) for macrophage migration inhibitory factor (MIF) has anti-proliferative activity in lung cancer cells. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34018657

Product Documents for MD13