MDM2/HDM2 [p Ser166] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05384

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MDM2/HDM2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S166 of human MDM2/HDM2 (NP_002383.2). TSSRR

Modification

p Ser166

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDM2/HDM2 [p Ser166] Antibody - BSA Free

Western Blot: MDM2/HDM2 [p Ser166] AntibodyBSA Free [NBP3-05384]

Western Blot: MDM2/HDM2 [p Ser166] AntibodyBSA Free [NBP3-05384]

Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
MDM2/HDM2 [p Ser166] Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 [p Ser166] Antibody - BSA Free [MDM2/HDM2] -

Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 [p Ser166] Antibody - BSA Free [MDM2/HDM2] - Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using MDM2/HDM2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for MDM2/HDM2 [p Ser166] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDM2/HDM2

MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Gene Symbol

MDM2

Additional MDM2/HDM2 Products

Product Documents for MDM2/HDM2 [p Ser166] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MDM2/HDM2 [p Ser166] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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