MG 132

Tocris Bioscience | Catalog # 1748

Proteasome and calpain inhibitor. Inhibits NF-κB activation
Tocris Bioscience

Key Product Details

Description

Proteasome and calpain inhibitor. Inhibits NF-κB activation

Alternative Names

Z-LLL-al,Z-Leu-Leu-Leu-CHO
View all Calpain Inhibitors »

Product Description

MG 132 is a potent cell-permeable inhibitor of proteasome (IC50 = 100 nM) and calpain (IC50 = 1.2 μM). MG 132 inhibits TNF-α-induced NF-κB activation and IκBα degradation. Induces neurite outgrowth in PC12 cells and has anticancer properties in vitro. MG 132 inhibits SARS-CoV-2 Mpro in vitro (IC50 = 3.9 μM) and inhibits viral replication. Also enhances adeno-associated viral transduction efficiency of human cell lines by around 50-fold.

Product Specifications for MG 132

Molecular Weight

475.63

Formula

C26H41N3O5

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-[(Phenylmethoxy)carbonyl]-L-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-L-leucinamide

CAS Number

133407-82-6

PubChem ID

90488707

InChI Key

AUQBNOXQYSNYAH-OJSMNCEXSA-N

SMILES

[H]C(C)C[C@H](NC(=O)OCC1=CC=CC=C1)C(=O)NC(CC(C)C)C(=O)N[C@@H](CC(C)C)C(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 47.56 100
Ethanol 47.56 100

Preparing Stock Solutions for MG 132

The following data is based on the product molecular weight 475.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.10 mL 10.51 mL 21.02 mL
5 mM 0.42 mL 2.10 mL 4.20 mL
10 mM 0.21 mL 1.05 mL 2.10 mL
50 mM 0.04 mL 0.21 mL 0.42 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Product Documents for MG 132

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MG 132

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Customer Reviews for MG 132 (5)

5 out of 5
5 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used MG 132?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 的 5 reviews Showing All
Filter By:
Clear Filters X
  • test
    Name: Chi Ngo
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: iPSC-derived neurons
    Verified Customer | Posted 09/06/2025
    MG132 used at the concentration of 1uM for 48h was toxic to human iPSC-derived neurons.
    Optimize the MG132 dose to use in human iPSC-derived neurons.
    MG 132 1748
  • Inhibition of NF-kB activation
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/13/2022
    MG 132 was used to block NF-kB activation and determine the pathway of a chemotherapeutic in pancreatic cancer lines. The inhibitor worked nicely, and the results were consistent
  • MG132
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: H9C2
    Verified Customer | Posted 05/28/2021
    Treated H9C2 cells for 6hours to inhibit ubiquitination.
    MG 132 1748
  • MG-132 (15 µM)
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: LP-1 cell line
    Verified Customer | Posted 12/11/2019
    Cells were treated with MG-132 (15 µM) for 18 hours in the absence or presence of GSK2656157 (10 µM)
    MG 132 1748
  • MG 132 Usage Information
    Name: Chao Li
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human intestine subepithelial myofibroblasts
    Verified Customer | Posted 01/08/2018
    It works well and dissolves perfectly in DMSO. I treated the cells with MG 132 (final concentration: 10 micromolar) in serum free medium for 24 hours.

There are no reviews that match your criteria.

Showing  1 - 5 的 5 reviews Showing All