Mouse CCL8/MCP-2 Antibody (146123)

R&D Systems | Catalog # MAB790

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 146123
View all CCL8/MCP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL8/MCP-2
Glu20-Pro97
Accession # Q9Z121

Specificity

Detects mouse CCL8/MCP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 3, 4, 5, 6, 7,  9/10MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CCL8/MCP-2 Antibody (146123)

Detection of kcqkq4kkgurcpytqqrxf.jpg by Immunocytochemistry/ Immunofluorescence

Detection of kcqkq4kkgurcpytqqrxf.jpg by Immunocytochemistry/ Immunofluorescence

Hypofractionated radiotherapy promotes M2-like Ccl8high macrophages infiltration and leads to poor prognosis. A Kaplan–Meier plots showing worse clinical prognosis in the LUAD and HNSC patients with the higher expression level of the Mac_Ccl8 signature. HR, hazard ratio. B Schematic diagram of the combination treatment with hypofractionated radiotherapy and the Bindarit. The intraperitoneally administration of Bindarit began from day 5 to day 11 post-tumor injection, and radiation treatment was initiated from day 7 to day 9 post-tumor injection. C Growth curves of tumors in LLC-bearing mice in the indicated treatment groups (n = 5 mice/group). Data are presented as mean ± SD with two-way ANOVA test. D Representative examples in the indicated treatment groups of multiplex immunofluorescent labeling F4/80, CD206 and CCL8. Yellow, F4/80, Green, CD206; Red, CCL8; Blue, DAPI. E Percentages of M1 and M2 macrophages in the specific treatment groups analyzed by flow cytometry (n = 3/group). F Representative flow cytometry panels showing M2 macrophages (top) and M1 macrophages (bottom). BI, Bindarit; RT, Radiation therapy; RT + BI, the combination therapy of the radiation and the Bindarit Image collected and cropped by CiteAb from the following open publication (https://translational-medicine.biomedcentral.com/articles/10.1186/s1296…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL8/MCP-2 Antibody (146123)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL8/MCP‑2

Reviewed Applications

Read 2 reviews rated 3.5 using MAB790 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL8/MCP-2

CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Alternate Names

MCP-2, MCP2

Entrez Gene IDs

6355 (Human); 20307 (Mouse)

Gene Symbol

CCL8

UniProt

Q9Z121

Additional CCL8/MCP-2 Products

Product Documents for Mouse CCL8/MCP-2 Antibody (146123)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL8/MCP-2 Antibody (146123)

For research use only

Citations for Mouse CCL8/MCP-2 Antibody (146123)

Customer Reviews for Mouse CCL8/MCP-2 Antibody (146123) (2)

3.5 out of 5
2 Customer Ratings
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  • Mouse CCL8/MCP-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 03/30/2025
    Mouse CCL8/MCP-2 Antibody (146123) MAB790
  • Mouse CCL8/MCP-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Verified Customer | Posted 01/24/2024
    Mouse CCL8/MCP-2 Antibody (146123) MAB790

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