Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Western Blot, Neutralization, Immunocytochemistry, ELISA Capture, Inhibition

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 295108
View all Complement Component C5a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Complement Component C5a
Asn679-Arg755
Accession # P06684

Specificity

Detects mouse Complement Component C5a in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Complement Component C5a Antibody

N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody.

N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody.

Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl- beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Complement Component C5a Monoclonal Antibody (Catalog # MAB21501). The ND50 is typically 0.1-0.5 µg/mL in the presence of cytochalasin-B.

Applications for Mouse Complement Component C5a Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Complement Component C5a (Catalog # 2150-C5)

Neutralization

Measured by its ability to neutralize Complement Component C5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Complement Component C5a and cytochalasin‑B.

Mouse Complement Component C5a Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Complement Component C5a Biotinylated Antibody (Catalog # BAF2150)
  • Standard: Recombinant Mouse Complement Component C5a Protein (Catalog # 2150-C5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3 using MAB21501 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C5a

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

References

  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.

Alternate Names

C5, C5A, HE

Entrez Gene IDs

727 (Human); 15139 (Mouse)

Gene Symbol

C5

UniProt

P06684

Additional Complement Component C5a Products

Product Documents for Mouse Complement Component C5a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Complement Component C5a Antibody

For research use only

Citations for Mouse Complement Component C5a Antibody

Customer Reviews for Mouse Complement Component C5a Antibody (2)

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2 Customer Ratings
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  • Mouse Complement Component C5a Antibody
    Name: William Jackson
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Skin tissue
    Species: Mouse
    Verified Customer | Posted 11/12/2018
    Diffuse staining also present in C5 KO. OCT embedded frozen tissue, acetone fixed. 1/500 primary concentration with a 1/400 goat anti-rat IgG AlexaFluor555 secondary.
    Mouse Complement Component C5a Antibody MAB21501
  • Mouse Complement Component C5a Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 10/10/2018

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