Mouse GDF-1 Propeptide Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3069V Datasheet / COA / SDS

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AF3069V-100UG

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Mouse GDF-1 Propeptide Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF-1 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant mouse (rm) GDF-1 and rmGDF-3 Propeptide is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GDF-1 Propeptide
Pro25-Leu235
Accession # P20863

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GDF-1

Mouse GDF-1 Propeptide is a 26 kDa, secreted monomeric glycopeptide. It is synthesized as part of a 357 amino acid (aa) preprocursor that contains a 23 aa signal sequence, a 214 aa Propeptide and a 120 aa mature molecule. Following secretion, the proregion is presumably proteolytically cleaved from the mature molecule at an Arg-Arg dibasic residue motif. The amino acid sequence of mouse GDF-1 Propeptide is 65% and 78% identical to that of human and rat GDF-1 Propeptide, respectively.

Long Name

Growth Differentiation Factor 1

Entrez Gene IDs

2657 (Human); 14559 (Mouse)

Alternate Names

DORV; DTGA3; embryonic growth/differentiation factor 1; GDF1; GDF-1; growth differentiation factor 1