Mouse GDF-7/BMP-12 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF779G Datasheet / COA / SDS

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AF779G-100UG

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All GDF-7/BMP-12 Antibodies

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Mouse GDF-7/BMP-12 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF-7 in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant mouse (rm) GDF-5 and rmGDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF-9

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse GDF-7 (R&D Systems, Catalog # 779-G7)
Thr316-Arg461
Accession # P43029

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GDF-7/BMP-12

Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta ) superfamily. Mature GDF-7 contains 146 aa residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80 - 86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.

Long Name

Growth Differentiation Factor 7

Entrez Gene IDs

151449 (Human); 238057 (Mouse)

Alternate Names

BMP12; BMP-12; GDF7; GDF-7; growth differentiation factor 7; growth/differentiation factor 7